KRBP72 eCLIP and partially edited A6 in T. brucei brucei KRBP72 RNAi cells

PCF T. brucei KRBP72-PTP tagged KRBP72 RNAi cell lines were grown in the presence or absence of 4 ug/mL doxycycline for 3 days. RNA was isolated using Trizol followed by phenol/chloroform extraction and ethanol precipitation. RNA samples were treated with DNase and phenol/chloroform extracted and ethanol precipitated. Two biological replicate experiments were performed, and western blot was used to validate the level of KRBP72 knockdown. cDNA was generated from DNase-treated RNA using gene-specific primers.