Remote ischemic conditioning protects against testicular ischemia∕reperfusion injury in rats

Abstract Purpose To evaluate the effect of remote ischemic conditioning associated to N-acetylcysteine (NAC) on testicular ischemia∕reperfusion (I∕R) injury in rats. Methods Twenty-five adult male Wistar rats were randomly distributed into five experimental groups (n=5), as follows: Sham, I∕R, Perconditioning (PER), NAC and PER+NAC. Two-hour ischemia was induced by rotating the left testis 720° to clockwise direction, followed by 4 hours of reperfusion. Perconditioning was performed by three I/R cycles of 10 min each on the left limb, 30 min before reperfusion. N-acetylcysteine (150 mg∕kg) was administered 30 min before reperfusion. Results Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was statistical difference between PER and Sham, and PER+ NAC groups (p<0.05) in plasma. Conclusions The protective effect of perconditioning isolated in the reduction of lipid peroxidation related to oxidative stress was demonstrated. However, when Perconditioning was associated with NAC, there was no protective effect against testicular injury of ischemia and reperfusion.

摘要：目的 评估联合应用N-乙酰半胱氨酸（N-acetylcysteine, NAC）的远程缺血预处理（remote ischemic conditioning）对大鼠睾丸缺血再灌注（ischemia/reperfusion, I/R）损伤的影响。方法 将25只成年雄性Wistar大鼠随机分为5个实验组（每组n=5），分别为假手术组（Sham）、缺血再灌注组（I/R）、预处理组（Perconditioning, PER）、NAC组及PER+NAC联合组。通过将左侧睾丸顺时针旋转720°构建2小时缺血模型，随后实施4小时再灌注。再灌注前30分钟，通过对左侧肢体进行3个周期、每个周期10分钟的缺血再灌注操作完成预处理干预；于再灌注前30分钟给予N-乙酰半胱氨酸（150 mg/kg）。结果 缺血再灌注组的血浆丙二醛（malondialdehyde, MDA）水平与其余各组均存在显著统计学差异（p<0.01）；此外，预处理组与假手术组、PER+NAC联合组的血浆MDA水平亦存在统计学差异（p<0.05）。结论 本研究证实，单独应用预处理可减轻氧化应激相关的脂质过氧化反应，发挥保护作用；但当预处理联合N-乙酰半胱氨酸使用时，未表现出对睾丸缺血再灌注损伤的保护效应。