[Functional evaluation of blood neutrophils in primiparous and pluriparous Lacaune ewes]

ABSTRACT This study aimed to evaluate the neutrophil oxidative metabolism and phagocytosis of Zymonsan particles of primiparous and pluriparous Lacaune ewes during the first 30 days after lambing. A total of 20 ewes were evaluated, 10 primiparous (GPR) and 10 pluriparous (GPL). Evaluation of basal oxidative metabolism was performed using the nitroblue tetrazolium (NBT) technique, stimulating neutrophil phagocytosis with Zymosan particles. Blood samples were collected at parturition day (M1) and 1, 3, 7, 15 and 30 days after parturition, corresponding to M2, M3, M4, M5 and M6, respectively. In relation to the groups, GPR presented lower oxidative basal metabolism neutrophils in M1 compared to M4, in M3 with M1, M2, M4 and M5. In M4 and M5 differences were found at all times and in M6 with M4 and M5. Higher percentage of neutrophils than phagocytes were found in M4, M5 and M6 than in M1 and M2 in GPL animals. At all times GPR presented a lower percentage of phagocytosis than GPL. Thus it is concluded that the immune response in pluriparous sheep was more efficient than in primiparous sheep.

摘要：本研究旨在评估产羔后前30天内，初产（primiparous）与经产（pluriparous）拉克纳母羊的中性粒细胞（neutrophil）氧化代谢（oxidative metabolism）及对酵母聚糖（Zymosan）颗粒的吞噬功能（phagocytosis）。本研究共纳入20只受试母羊，其中初产组（primiparous group, GPR）与经产组（pluriparous group, GPL）各10只。本研究采用硝基蓝四唑（nitroblue tetrazolium, NBT）技术检测中性粒细胞基础氧化代谢水平，并以酵母聚糖颗粒刺激中性粒细胞发生吞噬作用。分别于产羔当天（记为M1）及产羔后1、3、7、15、30天采集血液样本，对应时间点依次记为M2、M3、M4、M5及M6。组间比较结果显示：初产组（GPR）在M1时间点的中性粒细胞基础氧化代谢水平低于M4时间点，在M3时间点则显著低于M1、M2、M4及M5时间点；在M4与M5时间点，各采样时刻间均存在显著差异，M6时间点与M4、M5时间点亦存在显著差异。经产组（GPL）绵羊在M4、M5及M6时间点的中性粒细胞吞噬率均显著高于M1与M2时间点。所有采样时间点中，初产组（GPR）的中性粒细胞吞噬率均显著低于经产组（GPL）。综上，本研究得出结论：经产绵羊的免疫应答效率显著高于初产绵羊。