Genome-wide identification and characterization of long noncoding and circular RNAs in germline stem cells

Germline stem cells are germ cells at an early developmental stage, so their development is key to ensuring human reproduction and eugenics. There is increasing evidence that long noncoding RNA (lncRNA) and circular RNA (circRNA) play important roles in the development of germ cells. This data descriptor provides unique lncRNA and circRNA transcriptomic information for mouse germline stem cells. Using the Illumina HiSeq 2000 system, a total of 511,836,732 raw reads were generated. High-quality transcripts, lncRNAs and circRNAs were identificated and quantified using the reads, and more precise annotations of lncRNAs (especially 18573 novel lncRNAs) and circRNAs were performed in germline stem cells. We then analyzed the transcript structures, genetic variants and interaction between circRNA and microRNA to provide the basis for subsequent functional experiments.This comprehensive dataset should deepen our understanding of mouse germline stem cells, providing a theoretical foundation for research on germ cell development and human reproduction, among others.

生殖系干细胞（Germline Stem Cells）是处于发育早期阶段的生殖细胞，其发育是保障人类生殖与优生学的核心环节。越来越多的研究证据表明，长链非编码RNA（long noncoding RNA，lncRNA）与环状RNA（circular RNA，circRNA）在生殖细胞发育过程中发挥着重要作用。本数据集为小鼠生殖系干细胞提供了独有的长链非编码RNA与环状RNA转录组学数据。本研究依托Illumina HiSeq 2000测序平台，共生成511,836,732条原始读段。基于上述原始读段，研究人员完成了高质量转录本、长链非编码RNA与环状RNA的鉴定与定量分析，并对小鼠生殖系干细胞中的长链非编码RNA（尤其包含18573条全新长链非编码RNA）与环状RNA进行了更为精准的注释。随后，研究人员对转录本结构、遗传变异以及环状RNA与微小RNA（microRNA）的相互作用进行了分析，为后续功能实验提供了理论依据。本综合性数据集将加深学界对小鼠生殖系干细胞的认知，可为生殖细胞发育、人类生殖等相关领域的研究提供理论基础。