Diploid vcf

In total 380 potato clones were genotyped. Library construction and genotyping by genotyping by sequencing (GBS) was outsourced to the Genomics Facility at Cornell University in 2015. The DNA was digested with EcoT221 restriction enzyme and the libraries were 48x multiplexed for sequencing. The diploid calling was by the service provider using the Tassel pipeline (Bradbury et al., 2007).

本研究共对380个马铃薯克隆系进行了基因分型。2015年，本研究将通过测序分型（genotyping by sequencing, GBS）技术开展的文库构建与基因分型工作外包至康奈尔大学基因组学平台。实验所用DNA经EcoT221限制性内切酶消化，构建的文库采用48重多重混合方式以用于测序。二倍体基因型分型工作由服务提供商基于Tassel分析流程（Bradbury等人，2007年）完成。