Re-evaluating the systematics of Dendrolycopodium using RAD-sequencing Raw sequence reads. Lycopodiaceae

The genus Dendrolycopodium (Lycopodiaceae) includes four to five species across North America and East Asia. Species identification in Dendrolycopodium is difficult due to limited or incon-sistent characters. In addition, plants with intermediate morphologies regularly occur, potentially indica-tive of interspecific hybridization. To determine the species relationships in Dendrolycopodium and in-vestigate the existence of hybrids, we generated a draft genome assembly for D. obsurum and carried out double-digest restriction-site associated DNA sequencing (RADSeq) on 86 Dendrolycopodium specimens, including all described species and 11 individuals with intermediate morphology.