Tendon methylation microarray after particulate matter inhalation

Genome-wide DNA methylation profiles were determinen. DNA amplicons were prepared using Differential Methylation Hybridization (DMH) method, subsequently hybridized on to the Customized Agilent Rat CpG island Microarray. The goal was to unravel the DNA methylation patterns in different subgropus of tendon tissue according to partciulate matter exposure.本研究完成了全基因组DNA甲基化谱的测定。采用差异甲基化杂交（Differential Methylation Hybridization, DMH）方法制备DNA扩增子，随后将制备得到的扩增子与定制化安捷伦大鼠CpG岛微阵列进行杂交。本研究旨在阐明不同亚组肌腱组织在颗粒物暴露下的DNA甲基化模式。