Genetic and epigenetic variation among inbred littermates: identification of metastable epialleles in the mouse. Mus musculus

Genomic DNA was extracted from the livers of 5 22week old male mice, bisulfite converted and sequenced to ~30x coverage. The mice were obtained from two litters from an inbred agouti viable yellow colony. The individuals were isogenic but differed in their coat phenotype with one having a yellow coat, one a psuedoagouti coat and the remaining were phenotypically C57/BL6. The resulting DNA metylation profiles were investigated for individual differences in DNA methylation, including the identificaiton of novel metastable epialleles. Overall design: Whole genome bisulfite sequencing of liver DNA from five inbred male mice