Serial section TEM of touch receptor neurons from an transgenic alpha tubulin aceyltransferase (K40Q) experiment. mec-17;atat-2;mec-12 triple null

To test whether acetylation of MEC-12[K40] could account for the observed effects on microtubule quinary structure, we used our HPF-FS technique to analyze microtubules in transgenic mutants expressing WT and mutant MEC-12 iso- forms selectively in the touch receptor neurons.This data set confirmed that animals lacking mec-17;atat-2;mec-12 also lacked 15pf microtubules in the TRNS

为验证MEC-12[K40]的乙酰化是否可解释观测到的微管五级结构变化，我们采用高压冷冻-冷冻替代技术（High Pressure Freezing-Freeze Substitution, HPF-FS），对仅在触觉感受器神经元中特异性表达野生型（Wild Type, WT）与突变型MEC-12同工型的转基因突变体中的微管进行分析。本数据集证实，同时缺失mec-17、atat-2与mec-12的动物，其TRNS中亦不存在15原丝微管（15pf）。