Immunoglobulins G from Systemic Sclerosis Patients Modify the Proteome of Endothelial Cells Depending on Antinuclear Antibodies Serotypes

Systemic sclerosis (SSc) is a heterogeneous connective tissue disease characterized by autoimmunity, vasculopathy and excessive fibrosis (1). Antinuclear antibodies (ANA) are strong diagnostic and prognosis biomarkers but their pathogenicity in fibrosis and vasculopathy remains unclear. Functional antibodies (FA) such as angiotensin II type 1 receptor [AT1R] and endothelin-1 type A receptor [ETAR] have been described in SSc and might be implicated in vasculopathy, in which endothelial cells (EC) are key players.We aimed to explore the effect of purified IgG from SSc patients on the proteome of EC and examine the influence of ANA serotypes or FA. The detailed methodology is available in the Supplementary Material section.