Analysing the effect of cellular energy levels on codon-specific ribosome occupancy in vivo

The aim of this study is to evaluate how cellular energy levels shape codon-specific decoding and affect codon-mediated mRNA degradation. To this end we made use of HT5P-seq (Zhang & Pelechano, 2021, PMID:35474692) in S. cerevisiae to footprint the ribosome of co-translationally degraded mRNAs in vivo upon swift changes in the concentration of intracellular ATP and other energy metabolites. Overall design: HT5P-seq was performed in 5 different time points (3 replicates each time points), spanning 5 min before and 10 min after the addition of antimycin A to respiring yeast as described in Walther T et al. (PMID: 20087341)