sgRNAs used in this study and CRISPR targeting efficiency measured by TIDE analysis.
收藏Figshare2022-09-29 更新2026-04-28 收录
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Genomic DNA was harvested from CRISPR-targeted and non-targeted cells and the targeted locus was amplified by PCR (~200bp upstream and downstream of the predicted cut site), and sequenced via capillary reaction. TIDE analysis was performed on the sequence traces from control and targeted cells to estimate the percentage of alleles that had INDELs resulting from double-stranded break repair. (CSV)
创建时间：
2022-09-29
