Transcriptional profiling of roots and shoots from Brachypodium distachyon seedlings flown on the ISS

Most major cereal grain crops are monocots. Yet, most investigations of plant adaptation to the spaceflight environment have been carried out on the dicotyledonous model plant, Arabidopsis thaliana. It remains unknown whether the conclusions from such studies can be extrapolated to monocotyledonous plants. To fill this knowledge gap, seedlings of three different accessions of Brachypodium distachyon (Bd21, Bd21-3, and Gaz8), a model for temperate grasses including cereal crops, were grown on the International Space Station in the Veggie hardware and on the ground in matched conditions. To synchronize germination, seedlings were grown for 24 hours in red light. Then, seedlings were grown with green/blue/red light using a 24 hour photo period for 4 additional days. At this point, seedlings were harvested and placed in room temperature RNAlater for 24 hours before being moved to -80 C for storage and later dissection into roots and shoots. This dataset features ribodepleted RNA-seq data from the Bd21, Bd21-3 and Gaz8 accessions.

绝大多数主流谷类作物均为单子叶植物（monocots）。然而，当前针对植物适应航天环境的研究，大多以双子叶模式植物拟南芥（Arabidopsis thaliana）为实验对象。目前尚不清楚此类研究所得结论能否外推至单子叶植物。为填补这一知识空白，本实验以涵盖谷类作物的温带禾本科模式植物二穗短柄草（Brachypodium distachyon）的3个不同种质系（Bd21、Bd21-3及Gaz8）为材料，分别在国际空间站的Veggie植物培养装置与地面匹配对照条件下培养其幼苗。为同步萌发进程，幼苗先在红光条件下培养24小时；随后将幼苗置于绿、蓝、红复合光下，以24小时为光周期继续培养4天。培养结束后收获幼苗，先置于室温下的RNAlater溶液中静置24小时，随后转移至-80℃环境中保存，后续再将样品拆解为根系与地上组织两部分。本数据集包含来自上述3个二穗短柄草种质系的核糖体RNA去除型RNA测序（ribodepleted RNA-seq）数据。