Analysis of transcriptional changes in E. coli expressing mtSSBs
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https://www.ncbi.nlm.nih.gov/sra/SRP486510
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We conducted mRNA-seq to compare gene expression of E. coli BL21-AI expressing green fluorescent protein (GFP) and those expressing tardigrade (R. varieornatus) mitochondrial single-stranded DNA-binding protein (mtSSB). Heterologous expression of mtSSB in E. coli increased desiccation survival. To determine if endogenous stress response pathways are induced by expression of mtSSB, we measured transcriptional differences 4 hr after inducing protein expression in these bacteria and compared to controls expressing GFP.
创建时间：
2024-10-17
