Vibrio cholerae SAD2095 induced

SAD2095 (T2SS and T4 competence pili) = rpoS*, pilA S81C, lacZ::lacIq, comEA-mCherry, ΔluxO, Ptac-tfoX, Ptac-recA, ∆TCP::ZeoR, ∆MSHA::CarbR, ∆CTX::KanR, ∆pilT::TmR Ankur: "For the taxonomy ID - unfortunately the strain we sent does not have a taxonomy ID. So, you can use the generic one for V. cholerae which is "666". The strain we are working with is related to N16961, but is certainly not the same strain, so not sure if you would want to indicate that somehow? Just for reference - the strain we sent is E7946 (although it is obviously heavily modified with a number of mutations). "

SAD2095（针对Ⅱ型分泌系统（Type 2 Secretion System, T2SS）与IV型感受态菌毛（Type IV competence pili, T4 competence pili）构建）的基因型如下：rpoS*、pilA基因S81C位点突变、lacZ位点插入lacIq元件、comEA与mCherry融合表达、luxO基因敲除、Ptac启动子驱动tfoX基因表达、Ptac启动子驱动recA基因表达、TCP基因座被ZeoR抗性标记替换、MSHA基因座被CarbR抗性标记替换、CTX基因座被KanR抗性标记替换、pilT基因座被TmR抗性标记替换。Ankur留言:"关于分类学ID——遗憾的是我们寄送的菌株暂无专属分类学ID，因此可使用霍乱弧菌（Vibrio cholerae）的通用分类学ID"666"。我们当前研究的菌株与N16961菌株亲缘相关，但绝非同一株系，因此不确定是否需要就此注明？仅供参考：我们寄送的菌株为E7946（尽管其已通过多轮突变完成了大量改造）。"