Complete listing of the mutant mouse genes screened and the associated allele information

A subset of genes (29) were screened as 2 different alleles and for 1 gene a total of 3 alleles have been screened. The different alleles were generally tm1a and tm1b to discern if there was any difference after cre-mediated excision of the critical exon and targeting cassette (for details of the EUCOMM/KOMP-CSD tm1a/tm1b allele nomenclature refer to Skarnes et al 9). In some instances, one allele was a point mutation and the other targeting the whole gene. ‘Screened gene’ is the gene name as it appears in Data Record 2, followed by the ‘full allele name’, ‘allele superscript’ and ‘allele MGI ID’ (where a record exists). The ‘unique identifier’ is the internal colony prefix that was used for tracking and is specific to the gene and allele within the institute. In some instances, there are multiple colony prefixes for the same gene and allele – this occurred when additional mice were generated for testing outside of the high-throughput screening project. ‘MGI Gene/Marker ID’ allows tracking of the gene independent of any change in gene name for which a number have changed. The ‘current symbol’ is the most recent gene name and ‘synonym screened’ is where the gene has since been changed at MGI (and also internally) so allows for cross checking to the original data. The full current gene name and feature annotation is listed together with the chromosomal location of the gene and the Ensembl ID.

本次筛选的基因中，29个基因各携带2种不同等位基因，另有1个基因共筛选出3种等位基因。本次实验所用的等位基因多为tm1a与tm1b，用于探究经Cre介导切除关键外显子及靶向载体盒后是否存在表型差异（关于EUCOMM/KOMP-CSD的tm1a/tm1b等位基因命名规则的详细说明，请参阅Skarnes等人发表的第9篇文献）。部分实验中，其中一种等位基因为点突变型，另一种则针对整个基因进行靶向改造。"筛选基因（Screened gene）"指数据记录2中所标注的原始基因名称，其后依次为"完整等位基因名称"、"等位基因上标"及"等位基因小鼠基因组信息学（MGI）ID"（若存在对应记录）。"唯一标识符（unique identifier）"为机构内部用于追踪小鼠种群的专属前缀，仅对应本机构内的特定基因与等位基因。部分情况下，同一基因与等位基因会对应多个种群前缀，该情况多出现在高通量筛选项目之外，额外生成用于实验验证的小鼠时。"小鼠基因组信息学（MGI）基因/标记ID"可独立追踪基因，不受基因名称变更的影响——目前已有多个基因的名称发生过变更。"当前基因符号（current symbol）"为该基因的最新官方名称；"筛选同义词（synonym screened）"用于指代那些已在MGI数据库及机构内部完成名称变更的基因，可用于与原始数据开展交叉核对。完整的当前基因名称、特征注释，连同该基因的染色体位置及Ensembl ID一并列出。