STED image quality comparison images

fixed HeLa cell with tubulin cytoskeleton stained with Star635. The sample was imaged with Leica TCS SP5 STED, 100x/1.4 oil immersion objective (HCX PL APO CS), pinhole 1.0 AU, excitation wavelength 635 nm. The depletion laser wavelength was set to 765 nm and its intensity was varied between 50-100\% -- the same field of view was imaged sequentially with two different detectors. The image size is 700x700 and pixel size 15x15 nm.

微管细胞骨架经Star635染料染色的固定海拉（HeLa）细胞。该样本采用徕卡TCS SP5受激损耗荧光显微镜（STED）进行成像，搭配100倍/1.4数值孔径油浸物镜（HCX PL APO CS），针孔尺寸为1.0爱里单位（AU），激发波长为635纳米。损耗激光波长设置为765纳米，强度在50%至100%范围内调节；同一视场经两个不同探测器依次完成成像。图像分辨率为700×700像素，像素尺寸为15×15纳米。