five

ARDRA based OTU classification.

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https://figshare.com/articles/dataset/_Response_of_Methanogens_in_Arctic_Sediments_to_Temperature_and_Methanogenic_Substrate_Availability_/1452196
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The number of positive clones produced from each sample is provided along with the frequency of OTUs for each clone library based on ARDRA analysis. OTUs were subsequently resolved into 5 family- level groups, based on analysis of 16S rRNA sequences from clone libraries i.e. Methanoregulaceae/WCHA2-08 group (OTU 1, 2, 3), the Methanospirillaceae group (OTU 4), Methanosarcinaceae group (OTU 5), Methanosaetaceae group (OTU 6, 7) and Candidatus ‘Methanoflorentaceae’ (Rice cluster II) group (OTU 8). *One positive clone had a unique ARDRA pattern but when sequence for this clone was obtained and analysed using Mallard [41] it was identified as a possible chimera and omitted from further analysis. (PDF)
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2015-06-17
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