RNA-seq data of 786-O cells transfected with control-sgRNA (Control) and PHF8-sgRNA (PHF8-KO)

RNA-seq analysis were applied to elucidate the transcriptional differences between 786-O cell lines transfected with control-sgRNA (Control) and PHF8-sgRNA (PHF8-KO). A total of 3575 differentially expressed genes (Fold Change > 2, or Fold Change＜0.5; FDR < 0.05) with 2097 down- and 1478 up-regulated genes were identified in PHF8-KO 786-O cells Total RNA was extracted from either PHF8 wild type or PHF8 knockout 786-O cells using Trizol reagent (CWBIO). After quality controlling and RNA library preparing, triplicate samples were subjected to sequence by Shanghai NovelBio Bio-Pharm Technology Co., Ltd (Shanghai, China). RNA-Seq reads were filtered and mapped to human genome (GRCh38, NCBI) utilizing HISAT2. HTSeq was used to calculate the gene count. Differentially expressed genes analysis was applied utilizing DESeq2 under following criteria: Fold Change > 2, or Fold Change＜0.5; FDR < 0.05.