Additional file 4 of Protein kinase A controls yeast growth in visible light

Additional file 4. Raw CDE data on the selected mutants for the confirmation assay #1 and there corresponding controls for day 0 and day 6 during light exposure and for day 0 and day 1 in the dark. The file includes the following datasheets: Sheet 1. “read me”, contains some general information about the data presented. Sheet 2: “ confirmation asay 1 - haploids”, contains the raw data and the calculations that are part of our scoring regime for the selected haploids. Sheet 3: “control strains_- haploids”: contains the raw data and the calculations that are part of our scoring regime for the control (his3∆) for the haploids indicating the median dark-normalized light-growth for the high-confidence range used for the normalization of the mutants (all control strains grew well even at low initial cell-densities and thus all represented the high-confidence range). Sheet 4: “ confirmation asay 1 - diploids”, contains the raw data and the calculations that are part of our scoring regime for the selected diploids. Sheet 5: “control strains - diploids”, contains the raw data and the calculations that are part of our scoring regime for the control (wild type, BY4743) indicating the median dark-normalized light-growth for each confidence range used for the normalization of the mutants.

附加文件4：验证实验#1中所选突变体的原始CDE数据，以及光照暴露下第0天、第6天和黑暗条件下第0天、第1天对应的对照样本数据。本文件包含以下数据表：表1："read me"，包含本次展示数据的相关通用说明；表2："验证实验1 - 单倍体"，包含所选单倍体评分体系所需的原始数据与计算结果；表3："对照菌株_单倍体"，包含单倍体对照（his3Δ）的评分体系所需的原始数据与计算结果，该对照的暗标准化光照生长中位数处于用于突变体标准化的高置信度区间内（所有对照菌株即使在初始细胞密度较低时仍可良好生长，因此均符合高置信度区间要求）；表4："验证实验1 - 二倍体"，包含所选二倍体评分体系所需的原始数据与计算结果；表5："对照菌株 - 二倍体"，包含二倍体对照（野生型BY4743）的评分体系所需的原始数据与计算结果，该数据用于获取用于突变体标准化的各置信度区间的暗标准化光照生长中位数。