Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast.

Data from ProteomeXchange, PXD ID: PXD001445. File: vagas_O1210_369.c.mzXML. Published as part of Mol Syst Biol. 2014 Dec 9;10(12):767 . From the Abstract: {{i}} Cells respond to environmental stimuli via specialized signaling pathways. Concurrent stimuli trigger multiple pathways that integrate information, predominantly via protein phosphorylation. Budding yeast responds to NaCl and pheromone via two mitogen-activated protein kinase cascades, the high osmolarity, and the mating pathways, respectively. To investigate signal integration between these pathways, we quantified the time-resolved phosphorylation site dynamics after pathway co-stimulation. Using shotgun mass spectrometry, we quantified 2,536 phosphopeptides across 36 conditions ... {{/i}}