Genome Sequencing of Filovirus Stocks. Filoviridae

Ebola and Marburg viruses are closely related strains causing severe diseases in humans and nonhuman primates. The increased frequency of outbreaks of hemorrhagic fever caused by Ebola and Marburg in central and western Africa and the potential use of such agents as biological weapons underscore the need to have a better understanding of these viruses. As part of this project we will sequence stocks of various filovirus strains using multiple next-generation sequencing platforms. The rationale behind the project is to obtain gold standard complete genome consensus sequences for each of these stocks and understand the level of any SNP variation within the virus population.