Sharma_2025

Using genome-wide association (GWA) and quantitative trait loci (QTL) mapping, we identified a major QTL on chromosome 8 containing candidate genes encoding cation/H⁺ exchangers (CHX), which are involved in ion transport and homeostasis. To validate these findings, we analyzed a mapping population derived from <i>V. acerifolia</i><i> </i>longii 9018 and the commercial rootstock GRN3, confirming the chromosome 8 locus as a major determinant of chloride exclusion. Structural variant analysis revealed key non-synonymous substitutions within CHX genes, suggesting potential functional roles in salinity tolerance. Additionally, we discovered a novel QTL on chromosome 19 enriched with G-type lectin S-receptor-like serine/threonine-protein kinases, known regulators of stress signaling. By integrating extensive phenotypic and genomic data across a diverse <i>Vitis</i> collection, this study provides novel insights into the genetic architecture of chloride exclusion and identifies valuable candidate genes for breeding salt-tolerant rootstocks.

本研究借助全基因组关联分析（genome-wide association, GWA）与数量性状位点（quantitative trait loci, QTL）定位技术，在8号染色体上鉴定到一个主效数量性状位点，其包含编码阳离子/H⁺转运蛋白（cation/H⁺ exchangers, CHX）的候选基因，这类蛋白参与离子转运与细胞稳态维持。为验证上述研究发现，我们分析了以V. acerifolia longii 9018与商业砧木GRN3为亲本构建的作图群体，证实该8号染色体位点是氯离子排除能力的核心遗传决定因子。结构变异分析显示，CHX基因内部存在关键非同义替换，提示其在植株耐盐性中可能发挥功能性作用。此外，本研究在19号染色体上发现了一个全新的数量性状位点，该位点富集G型凝集素S受体样丝氨酸/苏氨酸蛋白激酶，这类激酶是应激信号通路的已知调控因子。通过整合多样化葡萄属（Vitis）种质资源的海量表型与基因组数据，本研究为氯离子排除的遗传架构提供了全新见解，并为耐盐砧木育种鉴定出极具价值的候选基因。