Dataset: Floral presence and flower identity alter cereal aphid endosymbiont communities on adjacent crops

Floral plantings adjacent to crop fields can recruit populations of natural enemies by providing flower nectar and non-crop prey to increase natural pest regulation. Observed variation in success rates might be due to changes in the unseen community of endosymbionts hosted by many herbivorous insects, of which some can confer resistance to natural enemies, e.g. parasitoid wasps. Reduced insect control may occur if highly protective symbiont combinations increase in frequency via selection effects, and this is expected to be stronger in lower diversity systems.
We used a large-scale field trial to analyse the bacterial endosymbiont communities hosted by cereal aphids (Sitobion avenae) collected along transects into strip plots of barley plants managed by either conventional or integrated (including floral field margins and reduced inputs) methods. In addition, we conducted an outdoor pot experiment to analyse endosymbionts in S. avenae aphids collected on barley plants that were either ..., Field data were collected from The CSC platform at Balruddery Farm near Dundee, Scotland in 2016 and 2017. Conventional and integrated management treatments were randomly allocated to each half, and (for the barley fields) in each field, half three different barley varieties were planted as plot strips. Sitobion avenae aphids were collected on 6th July 2016 and 3rd July 2017, at four distances (5, 15, 30, 50 m) from the edge of the field into each barley variety strip. Aphids were stored in 70% ethanol and shipped to Technical University of Munich for symbiont analysis. At each sampling location (distance into the field strip), a maximum of five aphids (one per colony) were collected from three adjacent infested tillers in every barley strip (variety); aphids reproduce asexually, and females deposit a group of offspring in one patch before moving away, this approach minimises the chance of collecting aphids from the same clonal mother. Total aphid number was also counted for these three..., The Excel file contains an explanation of each of the variables and the two datasets in three worksheets:
(1) ReadME with variable explanations
(2) DATA_PotExperiment
(3) DATA_FIeldExperiment,

作物田块邻近的花卉种植带可通过提供花蜜与非作物猎物，招募天敌种群，从而增强自然虫害调控能力。观测到的防控成功率差异，可能源于诸多植食性昆虫所携带的未被关注的内共生菌（endosymbionts）群落变化——其中部分内共生菌可赋予宿主对天敌（如寄生蜂）的抗性。若高保护性共生菌组合通过选择效应提升其在种群中的频率，则可能导致虫害防控效果下降，且这种效应在生物多样性更低的生境中更为显著。

本研究依托大规模田间试验，分析了沿样带采集的麦长管蚜（*Sitobion avenae*）体内携带的细菌内共生菌群落；这些麦长管蚜取自采用常规管理或整合管理（含花卉田埂与减量化投入模式）的大麦条带样地。此外，本研究还开展了室外盆栽试验，分析采集于大麦植株上的麦长管蚜体内的内共生菌，供试大麦植株分为……。田间数据采集于2016至2017年，地点为苏格兰邓迪附近的Balruddery农场的CSC平台。试验中，每块田块均被随机划分为两半，分别设置常规管理与整合管理处理；针对大麦田，每块田内还种植了3个不同的大麦品种，以条带样地形式布设。分别于2016年7月6日与2017年7月3日采集麦长管蚜，采样点设置在距田块边缘不同距离（5、15、30、50米）的各大麦品种条带样地中。采集的蚜虫保存于70%乙醇中，随后运往慕尼黑工业大学开展共生菌分析。在每个采样点（即条带样地内的不同距离处），从每个大麦品种条带的3个相邻受害分蘖上最多采集5头蚜虫（每蚜群1头）；由于麦长管蚜行无性繁殖，雌蚜会在一处斑块内产下一批后代后再迁移，因此该采样方式可最大程度降低采集到同一克隆母株后代的概率。同时统计上述3个受害分蘖的蚜虫总数量。本数据集的Excel文件包含各变量说明与两份试验数据，共分为3个工作表：
1. 变量说明文档（ReadME）
2. 盆栽试验数据集（DATA_PotExperiment）
3. 田间试验数据集（DATA_FieldExperiment）