Additional file 8 of Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice

Additional file 8: Figure S2. (A) Volcano plot illustrating differentially expressed genes (P<0.05) in Trem2Y38C/Y38C versus Trem2-/- cortices. (B-C) Pathway enrichment of downregulated and upregulated genes (FDR<0.05) by Enrichr. (B) Pathway enrichment for downregulated genes indicates alteration in circadian rhythm associated pathways in Trem2Y38C/Y38C as compared to Trem2-/- mice (C) Upregulated genes show enrichment in pathways associated with protein processing in endoplasmic reticulum in Trem2Y38C/Y38C versus Trem2-/-. (B-C) P values are indicated at the end of each colored bar. Shaded gets lighter with smaller P value. Red bars represent enrichment for downregulated genes and green bars represent enrichment for upregulated genes. Sample sizes: WT mice, N = 8 (4 males, 4 females); Trem2Y38C/Y38C mice, N = 8 (4 males, 4 females); Trem2-/- mice, N = 5 (1 male, 4 females).

补充文件8：图S2。(A) 火山图展示了Trem2Y38C/Y38C小鼠与Trem2-/-小鼠大脑皮层中的差异表达基因（P<0.05）。(B-C) 采用Enrichr工具对下调基因与上调基因进行通路富集分析（错误发现率FDR<0.05）。(B) 下调基因的通路富集结果显示，相较于Trem2-/-小鼠，Trem2Y38C/Y38C小鼠的昼夜节律相关通路存在异常改变。(C) 上调基因则在Trem2Y38C/Y38C小鼠与Trem2-/-小鼠的对比组中，富集于内质网蛋白质加工相关通路。(B-C) 各彩色条形图的末端标注了对应P值，条形颜色深浅随P值减小而逐渐变浅；其中红色条形代表下调基因的富集结果，绿色条形代表上调基因的富集结果。样本量信息：野生型（Wild Type, WT）小鼠，N=8（雄性4只，雌性4只）；Trem2Y38C/Y38C小鼠，N=8（雄性4只，雌性4只）；Trem2-/-小鼠，N=5（雄性1只，雌性4只）。