Super Resolution analysis results from Slide 2-8

The following repository holds all of the analysis performed for the Manuscript titled "X-ray Lightsheet Microscopy: 3-Dimensional Localization of Non-Blinking Scintillating Nanoparticles with Nanometer Accuracy". The results were acquired with the use of the "Multi-Orientation MAXWELL" software, hosted on the following GitHub repository:https://github.com/SierraD/Multi-Orientation-Maxwell.The files include ThunderSTORM[1] results and protocol files obtained from analyzing the Tiff format images acquired via MAXWELL imaging for the sample "Slide 2-8" of microspheres coated with scintillating nanoparticles. The results files are CSV files, directly output from the ThunderSTORM software used to acquire the super resolution localizations. The ThunderSTORM parameters are found in the Protocol text files. The ThunderSTORM parameters were set to the default parameters, with changes made to the pixel size, which was set to 1 to deal with the anisotropic pixel size, as well as the fitting method, which was set to Maximum Likelihood Estimation to maximize the accuracy.[1] Ovesný, M. et al. ThunderSTORM; a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging. BioInformatics, Applications Note, 30 (16), 2389-2390 (2014).Please see the following GitHub repository for software and documentation for the Multi-Orientation MAXWELL analysis method:https://github.com/SierraD/Multi-Orientation-Maxwell/

本仓库包含题为《X射线光片显微镜（X-ray Lightsheet Microscopy）：基于纳米级精度的稳定闪烁纳米颗粒三维定位》的手稿所涉及的全部分析工作。本研究结果通过使用托管于下述GitHub仓库的"Multi-Orientation MAXWELL"软件获取：https://github.com/SierraD/Multi-Orientation-Maxwell。本次分析涉及的文件包括ThunderSTORM（ThunderSTORM）[1]结果文件与协议文件，均通过对MAXWELL成像获取的涂覆闪烁纳米颗粒的微球样品"Slide 2-8"的Tiff（Tiff）格式图像进行分析得到。结果文件为CSV（CSV）格式，直接由用于获取超分辨率定位结果的ThunderSTORM（ThunderSTORM）软件导出。ThunderSTORM（ThunderSTORM）的参数可在协议文本文件中查阅：本次分析采用默认参数，仅对两项参数进行了调整：一是将像素尺寸设为1，以解决各向异性像素尺寸问题；二是将拟合方法设置为最大似然估计（Maximum Likelihood Estimation），以最大化定位精度。[1] Ovesný, M. 等. ThunderSTORM（ThunderSTORM）：一款用于PALM（PALM）与STORM（STORM）数据分析及超分辨率成像的综合ImageJ（ImageJ）插件. 《生物信息学（BioInformatics）》应用笔记, 30(16), 2389-2390(2014)。有关Multi-Orientation MAXWELL（Multi-Orientation MAXWELL）分析方法的软件与文档，请参阅下述GitHub仓库：https://github.com/SierraD/Multi-Orientation-Maxwell/