Additional file 2 of Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

Additional file 2 Table S1. Two-way analysis of variance (ANOVA) table with p values (those less than 5% are highlighted in bold) and means squares (in italics) for response variables in two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P) or 0.25 mM P (High P); Zn, zinc. Table S2. Relative concentration of polar metabolites detected in root exudates of two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P) or 0.25 mM P (High P). * indicates metabolites for which fold change could not be calculated, as it was not detected in one of the treatments in both accessions. Table S3. Two-way analysis of variance (ANOVA) table with p values (those less than 5% are highlighted in bold) and means squares (in italics) for main root length and ANOVA on Ranks for lateral root length along the main root length, divided into quartiles, in two Brassica oleracea accessions (C6 and F103) grown in the high throughput root phenotyping system with Low phosphorus (P) and High P treatments (four groups in total) for 2 weeks. Table S4. All differentially expressed P-responsive genes and their expression in FPKM, in roots of two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P; LP) and 0.25 mM P (High P; HP). Differentially expressed genes were defined by comparing the gene expression in the Low P treatment with the gene expression in the High P treatment for each of the B. oleracea accession. Table S5. Gene ontology enrichment analysis for all differentially expressed genes in roots of two Brassica oleracea accessions (the C6 and the F103) grown in solutions containing 0.025 mM P (Low P) and 0.25 mM P (High P) for 2 weeks (n = 3 for each accession and each treatment). Bonferroni corrected p-values were used to determine significantly enriched categories and a corrected p-value of 0.05 was used as a cut-off. Table S6. P-responsive genes with orthologues in Arabidopsis thaliana. The table shows the gene expression of all P-responsive genes in the roots of two Brassica oleracea accessions (C6 and F103) grown hydroponically for 2 weeks in solutions containing 0.025 mM P (Low P) and 0.25 mM P (High P) which have A. thaliana ortholouges. Table S7. Shoot fresh weight, phosphorus (P) and zinc (Zn) concentrations and P efficiency ratio (calculated as Yieldlow / (Plow × Yieldlow) or Yieldhigh / (Phigh × Yieldhigh)) of the two Brassica oleracea accessions (C6 and F103) grown in a peat-based compost amended with Low P (5.25 mg L− 1) and High P (15.75 mg L− 1) used in the experiments. Data for fresh weight, shoot P concentration and P efficiency ratio are from [1] and shoot Zn concentrations are from [2]; DM, dry matter.

附加文件2 表S1：针对两份甘蓝（Brassica oleracea）种质（C6和F103）的响应变量构建的双因素方差分析（analysis of variance, ANOVA）表，其中包含p值（p值小于5%者以粗体标注）与均方（means squares，以斜体标注）。供试材料采用水培法种植2周，培养液分别添加0.025 mM磷（低磷，Low P）或0.25 mM磷（高磷，High P）；Zn为锌元素。
表S2：两份甘蓝（Brassica oleracea）种质（C6和F103）的根系分泌物中检测到的极性代谢物相对浓度。供试材料采用水培法种植2周，培养液分别添加0.025 mM磷（低磷，Low P）或0.25 mM磷（高磷，High P）。*标注的代谢物无法计算倍数变化，因其在两份种质的任一处理组中均未被检测到。
表S3：针对两份甘蓝（Brassica oleracea）种质（C6和F103）构建的双因素方差分析（ANOVA）表，包含主根长度的p值（p值小于5%者以粗体标注）与均方（以斜体标注），以及基于四分位数划分主根长度后对侧根长度进行的秩转换方差分析（ANOVA on Ranks）。供试材料采用高通量根系表型分型系统种植，设置低磷（Low P）与高磷（High P）处理（共4组），种植时长为2周。
表S4：两份甘蓝（Brassica oleracea）种质（C6和F103）的根系中所有磷响应差异表达基因及其每百万片段转录本数（fragments per kilobase of transcript per million mapped reads, FPKM）表达量。供试材料采用水培法种植2周，培养液分别添加0.025 mM磷（低磷，Low P；LP）或0.25 mM磷（高磷，High P；HP）。差异表达基因的定义为：针对每份甘蓝种质，比较其低磷处理与高磷处理的基因表达水平所得的差异基因。
表S5：针对两份甘蓝（Brassica oleracea）种质（C6与F103）的根系中所有差异表达基因开展的基因本体（Gene Ontology, GO）富集分析。供试材料采用添加0.025 mM磷（低磷，Low P）或0.25 mM磷（高磷，High P）的培养液水培种植2周，每份种质的每个处理组设置3次生物学重复（n = 3）。采用邦费罗尼校正（Bonferroni corrected）后的p值筛选显著富集的功能类别，校正后p值0.05作为显著性阈值。
表S6：在拟南芥（Arabidopsis thaliana）中存在直系同源基因的磷响应基因集。该表格展示了两份甘蓝（Brassica oleracea）种质（C6和F103）的根系中所有磷响应基因的表达量，供试材料采用水培法种植2周，培养液分别添加0.025 mM磷（低磷，Low P）或0.25 mM磷（高磷，High P），且这些基因在拟南芥中存在直系同源基因。
表S7：两份甘蓝（Brassica oleracea）种质（C6和F103）的地上部鲜重、磷（P）与锌（Zn）浓度，以及磷效率比值（计算公式为Yieldlow / (Plow × Yieldlow) 或 Yieldhigh / (Phigh × Yieldhigh)）。供试材料种植于添加了低磷（5.25 mg L−1）与高磷（15.75 mg L−1）的泥炭基堆肥中，为实验所用材料。鲜重、地上部磷浓度与磷效率比值的数据来自文献[1]，地上部锌浓度的数据来自文献[2]；DM为干物质（dry matter）。