Microsatellite genotypic data of Euptelea pleiospermum in STRUCTURE format

We sampled leaves from 678 individuals in 21 natural populations (30-36 individuals per population), covering the entire distribution of Euptelea pleiospermum in China.Total DNA was isolated from about 50 mg powdered leaf tissue following the protocol of a DNA extraction kit (Tiangen Biotech Co., LTD., Beijing, China). We used seven fluorescence-labeled microsatellite loci (EP036, EP059, EP081, EP087, EP091, EP278 and EP294; Zhang et al., 2008) to genotype our 678 DNA samples.

本研究从覆盖领春木（Euptelea pleiospermum）中国全境自然分布的21个天然居群中，采集了678份个体叶片样本，每个居群包含30~36份个体样本。我们称取约50 mg研磨后的叶片粉末，按照天根生化科技（北京）有限公司 (Tiangen Biotech Co., LTD., Beijing, China) 提供的DNA提取试剂盒操作流程完成总DNA提取。随后选用7个荧光标记微卫星位点（EP036、EP059、EP081、EP087、EP091、EP278及EP294；Zhang等，2008）对上述678份DNA样本进行基因分型。