GENE CONSTRUCT FOR EXPRESSION OF RECOMBINANT PROTEIN CONTAINING LETTUCE UBIQUITIN PROMOTER
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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PROBLEM TO BE SOLVED: To provide techniques that cause no silencing of expressed genes in the expression of a target gene using plant cells, maintaining a stable expression level over the progeny, and can harvest seeds efficiently. SOLUTION: The recombinant protein is expressed in plant cells such as lettuce using a lettuce ubiquitin promoter and an Arabidopsis thaliana heat shock protein 18.2 terminator. SELECTED DRAWING: Figure 1
待解决的技术问题:提供一类可在利用植物细胞表达靶基因的过程中,不会引发已表达基因的沉默效应,能够在子代中维持稳定的表达水平,且可高效收获种子的技术。
技术方案:采用莴苣泛素启动子(lettuce ubiquitin promoter)与拟南芥热休克蛋白18.2终止子(Arabidopsis thaliana heat shock protein 18.2 terminator),在莴苣等植物细胞中表达重组蛋白。
所选附图:图1
提供机构:
国家林业和草原科学数据中心
创建时间:
2023-02-12



