RNA-seq shows genes enhanced in translation and transcription pathways in MKs and platelets from Slfn14fl/flPF4-Cre mouse model

To identify the mechanism through which SLFN14 may regulate megakaryopoiesis and platelet function, we performed RNA sequencing analysis to define alterations in gene activity arising as a result of conditionally knocking down Slfn14 in vivo. Bulk RNA-sequencing was performed on total RNA extracted from mature murine Slfn14fl/flPF4-Cre derived MKs and peripheral blood platelets and compared to Slfn14+/+PF4-Cre control MKs/platelets. All individual samples were run in triplicate and normalised before comparisons between each SLFN14 mutant.