Brn3a+, Islet-1+ and βIII-tubulin+ RGC counts in Group 2 radial retinal sections from Group 2a eyes (intact optic nerves) and Group 2b eyes (21 days after ONC).

Immunohistochemically stained 20-µm-thick radial sections of retina through the optic nerve head, stained for βIII-tubulin (green; Ai and Aii) or Brn3a (green) and Islet-1 (red; Aiii and Aiv), taken from Group 2a eyes (Ai and Aiii) and Group 2b eyes (Aii and Aiv) with the ganglion cell layer (GCL) labelled. All images are representative of the two images/section, four sections/retina, and six retinae prepared from six different animals/group. DAPI was used as a nucleus stain (scale bar: 15 µm). In (B), the number of Brn3a+, Islet-1+ and βIII-tubulin+ cells, in a 1 mm linear region of the GCL from Group 2a/2b eyes. Asterisks indicate significant difference at pblack lines indicate significant difference at p