Distinct coral reef habitat communities characterized by environmental DNA metabarcoding
收藏Research Data Australia2024-12-14 收录
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https://researchdata.edu.au/distinct-coral-reef-dna-metabarcoding/2589984
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eDNA metabarcoding were applied using the rRNA 18S Universal eukaryote assay to explore differences in community profiles between samples collected from the lagoon and reef slope habitats across the Ningaloo Coast World Heritage Area in Western Australia
In May 2019, near-surface (1–2 m depth) 120 seawater samples were collected at 14 different localities along Ningaloo Reef, from the backreef lagoon and the exposed reef slope.
For shallow lagoon samples, five replicate 1 L samples were collected at five different sites across the reef using Nalgene bottles by snorkellers (site depth
本研究采用基于18S核糖体RNA（rRNA）通用真核生物检测体系的环境DNA（eDNA） metabarcoding技术，旨在探究澳大利亚西部宁加卢海岸世界遗产地潟湖与礁坡生境采集样本的群落组成差异。
2019年5月，研究人员于宁加卢礁沿岸14个不同采样点位，从礁后潟湖与裸露礁坡区域采集了120份近表层（水深1~2米）海水样本。
针对浅潟湖样本，浮潜人员使用耐洁（Nalgene）采样瓶于礁区5个不同采样点采集了5份平行1升水样（采样点水深
提供机构：
Australian Ocean Data Network
