Table S1 - Interaction between Hydrogenase Maturation Factors HypA and HypB Is Required for [NiFe]-Hydrogenase Maturation

Constructs and Primers used. Desired DNA fragment was amplified by PCR the from genomic DNA using the corresponding primer pair, followed by restriction digestion to create compatible ends on both inserts and plasmids. DNA ligation was performed to generate the desired plasmid. Restriction enzyme recognition sites on the primers listed are in lower case letters. Quikchange mutagenesis was performed using the corresponding primers to generate the variant construct from the parental wild-type construct. Mutation sites that are not complementary to the parental construct on the primers are in lower case letters.
(DOC)