Chemometric analysis of selective polyphenolic groups in <i>Asparagus racemosus</i> (Shatavar) root extracts by traditional and supercritical fluid (CO₂) based extractions

<i>Asparagus racemosus</i> root extracts were prepared by supercritical fluid (CO₂), soxhlet, and maceration-based methods also with various pretreatments. Thereafter, these root extracts were analyzed by High-Performance Liquid Chromatography, along with the chemometric study of the disparate phenolic groups. Among these, supercritical fluid (CO₂) based extract has a larger number of polar compounds and the antioxidant activity (98.54 ± 0.22 <i>µ</i>M Trolox equivalent mg⁻¹). It also has the best cell viability (94.37 ± 1.12%) and insulin release (0.82 ± 1.12 ng mL⁻¹) on β-pancreatic RINm-5F cells whereas, the best extractive yield (75.80 ± 3.44% w/w) was observed for pretreated aqueous soxhlet-based extract.

本研究采用超临界流体（CO₂）、索氏提取法及浸渍法，并辅以多种预处理手段，制备了匍匐天门冬（Asparagus racemosus）的根提取物。随后，通过高效液相色谱法（High-Performance Liquid Chromatography）对上述提取物进行分析，并针对不同酚类组分开展化学计量学研究。在所有提取物中，超临界流体（CO₂）提取物所含极性化合物数量最多，其抗氧化活性可达（98.54 ± 0.22）μM Trolox当量·mg⁻¹；针对胰腺β细胞RINm-5F的实验显示，该提取物同时具备最优的细胞存活率（94.37 ± 1.12%）与胰岛素释放量（0.82 ± 1.12 ng·mL⁻¹）。而经预处理的水相索氏提取物则获得了最高的提取率，为（75.80 ± 3.44% w/w）。

搜集汇总

背景与挑战

背景概述

该数据集研究了Asparagus racemosus（Shatavar）根提取物中多酚类化合物的化学计量学分析，比较了超临界流体（CO2）提取、索氏提取和浸渍法等传统方法的效果。关键发现表明，超临界流体提取物在极性化合物含量、抗氧化活性和细胞生物活性方面表现最佳，而预处理水相索氏提取物则实现了最高的提取率。