核酸药物递送系统研究数据集

针对血液细胞难以转染和操控的难题，研发了对血液细胞具有良好转染效果的精胺化普鲁兰多糖载体（Ps）、阳离子化明胶微球等核酸载体，确立了一种通过与两亲性阳离子药物的联用实现了更高效溶酶体逃逸的转染策略；针对白血病细胞特殊的转染环境，揭示了血清浓度影响阳离子载体RNA干扰效率的机理，证明血清环境下影响RNA干扰效率的关键因素是Ps与血清蛋白的质量比(Pr/Ps)；并进一步揭示了纳米颗粒力学性质与其核酸递送效率之间的关系。数据量约为3.5 GBTo address the challenges of transfection and manipulation difficulties in blood cells, we developed nucleic acid carriers including sperminated pullulan (Ps) and cationized gelatin microspheres, which exhibit excellent transfection efficiency for blood cells. We established a high-efficiency transfection strategy that achieves enhanced lysosomal escape via combination with amphiphilic cationic drugs. Targeting the specific transfection microenvironment of leukemia cells, we revealed the mechanism underlying the effect of serum concentration on the RNA interference efficiency of cationic carriers, and demonstrated that the critical factor affecting RNA interference efficiency in serum environments is the mass ratio of Ps to serum proteins (Pr/Ps). Additionally, we further uncovered the correlation between the mechanical properties of nanoparticles and their nucleic acid delivery efficiency. The total dataset size is approximately 3.5 GB.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于核酸药物递送系统研究，针对血液细胞转染难题，开发了精胺化普鲁兰多糖载体等核酸载体及转染策略，并揭示了血清浓度影响RNA干扰效率的机理以及纳米颗粒力学性质与递送效率的关系。数据量约为3.5 GB，包含2734个文件，源自'铁基纳米材料类酶效应与急性髓系白血病诊疗技术'项目。