Identification of α-isopropylmalate synthase mutants capable to overproduce L-leucine in Corynebacterium glutamicum

Table 1 Relative activities of α-IPMS variant at different concentrations of L-leucine. Table 2 Relative activities of wild-type α-IPMS at different concentrations of L-leucine. Table 3 Production of L-leucine in strain ATCC 13032/leuA. Table 4 Production of L-leucine in strain ATCC 13032/ilvBNV. Table 5 Production of L-leucine in strain ATCC 13032/leuAV. Table 6 Production of L-leucine in strainATCC 13032/ilvBNV-leuAV. Table 7 Characterisation of α-IPMS variant and wild-type α-IPMS. Table 8 Production of L-leucine and L-valine in different recombination strains.

表1 不同L-亮氨酸浓度下α-异丙基苹果酸合酶（α-IPMS）变体的相对活性。表2 不同L-亮氨酸浓度下野生型α-异丙基苹果酸合酶的相对活性。表3 菌株ATCC 13032/leuA中L-亮氨酸的产量。表4 菌株ATCC 13032/ilvBNV中L-亮氨酸的产量。表5 菌株ATCC 13032/leuAV中L-亮氨酸的产量。表6 菌株ATCC 13032/ilvBNV-leuAV中L-亮氨酸的产量。表7 α-异丙基苹果酸合酶变体与野生型α-异丙基苹果酸合酶的表征。表8 不同重组菌株中L-亮氨酸与L-缬氨酸的产量。