AAV-mediated RNAi of traumatic brain injury-induced genes in the rat hippocampus

To address the hypothesis that silencing deleterious or protective injury-induced genes in the rat hippocampus will reduce or increase the numbers of injured hippocampal neurons, alter cellular pathways essential for neuronal function and improve or worsen functional outcome after traumatic brain injury (TBI), we evaluated the effects of silencing neuronal nitric oxide synthase (nNOS) and glutathione peroxidase-1 (GPx-1) expression in the injured rat hippocampus.为验证下述假说：在大鼠海马体中沉默损伤诱导的有害或保护性基因，可改变创伤性脑损伤（TBI）后受损海马神经元的数量（增加或减少）、影响神经元功能所必需的细胞通路，并对创伤性脑损伤后的功能结局产生改善或恶化作用，本研究评估了在损伤大鼠海马体中沉默神经元型一氧化氮合酶（nNOS）与谷胱甘肽过氧化物酶-1（GPx-1）表达所产生的效应。