Research Data: The role of optical projection in the analysis of membrane fluctuations

In the zipped folder "codes.zip" and sub-folders:

flickering.m : matlab class that contains all the functions to set up and perform the analysis of a video of a flickering vesicle, to retrieve the spectrum of its fluctuations.

example.m : a brief example script where the flickering.m class is used.

export_spectrum.m : a scripts that loads the output of a flickering analysis (e.g. of example.m) and converts it to a json file format, ready for the fitting routine.

DataAnalysis.nb : commented Mathematica notebook containing the fitting routine and an example of its usage.

LTable.nb : support Mathematica file

DataExample_X.json : example spectra readable by the Mathematica notebook.

In the zipped folder "Sample_Data.zip":

Examples of flickering confocal videos collected for the same vesicle at different pinhole size (and thus z-resolution, see paper). The videos are in tif format. The text file "Experimental" contains information on the pixel size, framerate, and pinhole size of each video.

在压缩文件夹"codes.zip"及其子文件夹中：

flickering.m：一款Matlab类（matlab class），集成了所有用于搭建并分析闪烁囊泡视频、提取其涨落光谱的功能函数。

example.m：一段简短的示例脚本，用于演示flickering.m类的使用方法。

export_spectrum.m：一款脚本工具，可加载闪烁分析（如example.m的分析结果）的输出内容，并将其转换为适配拟合流程的JSON（json）文件格式。

DataAnalysis.nb：带注释的Mathematica笔记本文件，包含拟合流程及其使用示例。

LTable.nb：配套的Mathematica辅助文件。

DataExample_X.json：可被该Mathematica笔记本读取的示例光谱文件。

在压缩文件夹"Sample_Data.zip"中：

包含同一囊泡在不同针孔孔径（对应不同轴向分辨率，详见论文）下采集的闪烁共聚焦视频示例。所有视频均为TIF（tif）格式。文本文件"Experimental"中记录了各视频的像素尺寸、帧率及针孔孔径信息。