qPCR and kit data.pzfx

Ulcerative colitis (UC) is characterized by chronic inflammation and ulceration of the intestinal inner lining, resulting in various symptoms. Sea buckthorn berries contain a bioactive compound known as sea buckthorn polysaccharide (SBP). However, the precise mechanisms underlying the impact of SBP on UC remain unclear. In this study, we investigated the effects of pretreatment with SBP on colitis induced by DSS. Our findings demonstrate that SBP pretreatment effectively reduces inflammation, oxidative stress, and intestinal barrier damage associated with colitis. To further elucidate the role of SBP-modulated gut microbiota in UC, we performed fecal microbiota transplantation (FMT) on DSS-treated mice. The microbiota from SBP-treated mice exhibits notable anti-inflammatory and antioxidant effects, improves colonic barrier integrity, and increases the abundance of beneficial bacteria, as well as enhancing SCFA production. Collectively, these results strongly indicate that SBP-mediated amelioration of colitis is attributed to its impact on the gut microbiota, particularly through the promotion of SCFA-producing bacteria and subsequent elevation of SCFA levels. This study provides compelling evidence supporting the efficacy of pre-emptive SBP supplementation in alleviating colitis symptoms by modulating the gut microbiota, thereby offering novel insights into the potential of SBP as a regulator of the gut microbiota for colitis relief

溃疡性结肠炎（Ulcerative colitis, UC）以肠道内层慢性炎症与溃疡为特征，进而引发多种临床症状。沙棘浆果中含有一种名为沙棘多糖（sea buckthorn polysaccharide, SBP）的生物活性成分，但目前沙棘多糖对UC的具体作用机制仍未明确。本研究探究了沙棘多糖预处理对葡聚糖硫酸钠（Dextran Sulfate Sodium, DSS）诱导的结肠炎的干预效果。研究结果显示，沙棘多糖预处理可有效减轻结肠炎相关的炎症反应、氧化应激及肠道屏障损伤。为进一步阐明沙棘多糖调控肠道菌群在UC中的作用，本研究对经DSS处理的小鼠实施了粪便菌群移植（Fecal Microbiota Transplantation, FMT）。结果表明，来自沙棘多糖干预小鼠的肠道菌群具有显著的抗炎与抗氧化活性，可改善结肠屏障完整性，增加有益菌丰度，并促进短链脂肪酸（Short-Chain Fatty Acid, SCFA）生成。综上，本研究结果充分证实，沙棘多糖对结肠炎的改善作用依赖于其对肠道菌群的调控，尤其是通过促进产SCFA菌的增殖并提升SCFA水平。本研究为预防性补充SBP通过调控肠道菌群缓解结肠炎症状提供了有力证据，也为SBP作为肠道菌群调节剂用于结肠炎治疗的潜在应用价值提供了全新的研究视角。