Single crystal x-ray diffraction data set for human GABARAP complexed with artificial peptide IM-2

Human GABARAP (gamma-aminobutyric acid receptor-associated protein) belongs to the human autophagy-related protein 8 (hATG8) family which is implicated in various aspects of macroautophagy and other vesicle trafficking pathways. The family consists of six homologues classified into two subfamilies, the GABARAP subfamily and the microtubule-associated protein 1 light chain 3 (MAP1LC3) subfamily containing three proteins each. The human GABARAP protein was co-crystallised with the synthetic, artificial peptide IM-2. The crystal selected for data collection was obtained using PEG 8000 and ammonium sulfate as precipitating agents in a sodium cacodylate buffer. Glycerol was added as cryo-protectant during crystal preparation. Single crystal x-ray diffraction data extending to a diffraction limit of 1.42 Å were obtained via a helical scan on beamline ID30A-3 at the European Synchrotron Radiation Facility (ESRF; Grenoble, France), using an x-ray wavelength of 0.968 Å. The crystal structure will reveal the binding mode of the artificial ligand IM-2 to the human GABARAP protein.

人类GABARAP（γ-氨基丁酸受体相关蛋白，gamma-aminobutyric acid receptor-associated protein）属于人类自噬相关蛋白8（human autophagy-related protein 8，hATG8）家族，该家族与巨自噬（macroautophagy）及其他囊泡转运通路的诸多环节密切相关。该家族共包含6种同源蛋白，可划分为两个亚家族：GABARAP亚家族与微管相关蛋白1轻链3（microtubule-associated protein 1 light chain 3, MAP1LC3）亚家族，每个亚家族各含3种蛋白。人类GABARAP蛋白与合成人工肽IM-2完成了共结晶。用于数据收集的晶体是以PEG 8000与硫酸铵作为沉淀剂，在二甲胂酸钠（sodium cacodylate）缓冲液中制备获得。晶体制备过程中添加甘油作为低温保护剂。通过欧洲同步辐射装置（European Synchrotron Radiation Facility, ESRF；法国格勒诺布尔）ID30A-3光束线的螺旋扫描，获取了衍射极限达1.42 Å的单晶体X射线衍射数据，所用X射线波长为0.968 Å。该晶体结构将阐明人工配体IM-2与人类GABARAP蛋白的结合模式。