Speciation of neodynium in the rhizobacteria pseudomonas putida : focus on the influence of exposure media and Nd complexes with pyoverdine

With a predicted world population of over 9 billion people in 2050 and rapid economic growth, the pressure on natural resources is exponentially increasing. It is the case for critical metals, including Rare Earth Elements (REEs), that are the heart of a number of strategic technologies for the environmental transition. In this context, bio-inspired technologies could bring neat alternative to traditional pyro-metallurgy. Extraction strategies using bacterial proteins specific to REEs have recently emerge. This project aims to study the Nd complexes formed by molecules identified in the bacteria model Pseudomonas putida, such as periplasmic protein and siderophore.The second objective is to characterize Nd speciation in P. putida whole cells and compare it with the speciation of soluble proteins, with P. putida cultures performed in different media.This project should help to understand the implication of different ligands in REE behavior in P. putida.

预计2050年全球人口将突破90亿，叠加经济快速增长，自然资源所承受的压力正呈指数级加剧。包括稀土元素（Rare Earth Elements, REEs）在内的关键金属亦面临此类压力——它们是支撑环境转型领域诸多战略技术的核心材料。在此背景下，仿生技术有望为传统火法冶金提供全新的替代路径。近年来，靶向稀土元素的细菌蛋白提取策略逐渐兴起。本项目旨在探究恶臭假单胞菌（Pseudomonas putida）模型中鉴定出的分子所形成的钕（Nd）配合物，例如周质蛋白与铁载体。第二项研究目标为表征恶臭假单胞菌全细胞内的钕化学形态，并将其与可溶性蛋白的钕化学形态进行对比；实验将在不同培养基中培养恶臭假单胞菌。本项目将有助于阐明不同配体在恶臭假单胞菌体内稀土元素行为中的作用机制。