CRISPR screen analysis of human cardiomyocyte polyploidization and CCNB1 expression. CRISPR screen analysis of human cardiomyocyte polyploidization and CCNB1 expression
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA614515
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资源简介：
We characterize genetic determinants of human cardiomyocyte polypolidization and CCNB1 insufficiency using pooled CRISPR screen methods Overall design: CCNB1-GFP iPSCs were transduced with lentiviral Cas9 and libraries of sgRNA to generate pools of single-gene knockout cells, which were differentiated into cardiomyocytes, FACS sorted by ploidy and GFP status, and sequenced to assess gRNA representation
创建时间：
2020-03-23
