Improved control of Septoria tritici blotch in durum wheat using cultivar mixtures

Mixtures of cultivars with contrasting levels of resistance can suppress infectious diseases in wheat, as demonstrated in numerous field experiments. Most studies focused on airborne pathogens in bread wheat, while splash-dispersed pathogens have received less attention, and no studies have been conducted in durum wheat. We conducted a two-year field experiment in Tunisia, to evaluate the performance of cultivar mixtures with varying proportions of resistance (0–100%) in controlling the polycyclic, splash-dispersed disease Septoria tritici blotch (STB) in durum wheat. To measure STB severity, we used a high-throughput method based on digital image analysis of 3074 infected leaves collected from 42 and 40 experimental plots during the first and second years, respectively. This allowed us to quantify pathogen reproduction on wheat leaves and to acquire a large dataset that exceeds previous studies with respect to accuracy and precision. Our analyses show that introducing only 25% of a dis...

诸多田间试验已证实，选用抗性水平存在差异的小麦品种混播，可有效抑制小麦传染性病害。既往多数研究聚焦于普通小麦（bread wheat）的气传病原菌，而对飞溅传播型病原菌的关注相对不足，且目前尚无针对硬粒小麦（durum wheat）的相关研究。本研究于突尼斯开展了为期两年的田间试验，旨在评估不同抗性品种占比（0%~100%）的混播组合对硬粒小麦上多循环飞溅传播病害——小麦壳针孢叶斑病（Septoria tritici blotch, STB）的防控效果。为量化STB病害严重程度，本研究采用了基于数字图像分析的高通量检测方法：试验第一年与第二年分别从42个和40个试验小区采集了3074份染病小麦叶片用于分析。该方法可实现小麦叶片上病原菌繁殖量的定量检测，且本研究获取的大型数据集在准确性与精密性上均优于既往同类研究。本研究分析结果显示，仅引入25%的[原文未完整表述内容]