Additional file 1 of Identification, evolution, and expression of GDSL-type Esterase/Lipase (GELP) gene family in three cotton species: a bioinformatic analysis

Additional file 1: Supplementary Table 1.  The Gossypium genome sources used in this study. Supplementary Table 2. The orthologous/paralogous gene pairs of GELPs of G. raimondii, G. arboreum, and G. hirsutum. Supplementary Table 3. The Nucleic acid coding region and amino acid sequences of GELP genes of G. hirsutum. Supplementary Table 4. Analysis of the physical and chemical properties of GELP gene sequences in G. raimondii, G. arboreum, and G. hirsutum. Supplementary Table 5. Prediction analysis of the protein conserved structure and potential functional classification of the members of the GELP gene family in G. raimondii, G. arboreum, and G. hirsutum. Supplementary Table 6. The GELP homologous gene pairs that are collinear on each subgenome of G. raimondii, G. arboreum, and G. hirsutum. Supplementary Table 7. The rate of base substitution between sequences of GELP orthologous or paralogous gene pairs in G. raimondii, G. arboreum, and G. hirsutum. Supplementary Table 8. The FPKM value of the expression level of GELP genes specifically expressed in different tissues in G. raimondii, G. arboreum, and G. hirsutum. Supplementary Table 9. The FPKM value of the expression of GELP genes during the fiber initiation and fiber growth in G. hirsutum. Supplementary Table 10. The FPKM value of GELP genes expression in different tissues during seed germination in G. hirsutum. Supplementary Table 11. The FPKM value of expression of GELP genes in response to abiotic stress in G. hirsutum. Supplementary Table 12. The FPKM value of expression of GELP genes in response to stress from Verticillium dahliae in G. hirsutum. Supplementary Table 13. Up-regulated and down-regulated expressed GELP genes at the initial stage of fiber development. Supplementary Table 14. Up-regulated and down-regulated expressed GELP genes during fiber development. Supplementary Table 15. Up-regulated and down-regulated expressed GELP genes during the initial stage of seed germination. Supplementary Table 16. Up-regulated and down-regulated expressed GELP genes in root tissues after seed germination. Supplementary Table 17. Up-regulated and down-regulated expressed GELP genes in cotyledons after seed germination. Supplementary Table 18. Up-regulated and down-regulated expressed GELP genes in response to 20% PEG stress. Supplementary Table 19. Up-regulated and down-regulated expressed GELP genes in response to 4℃ low temperature stress. Supplementary Table 20. Up-regulated and down-regulated expressed GELP genes in response to 38℃ high temperature stress. Supplementary Table 21. Up-regulated and down-regulated expressed GELP genes after 400 mM NaCl stress treatment. Supplementary Table 22. Up-regulated and down-regulated expressed GELP genes in response to Verticillium dahliae. Supplementary Table 23. Statistics of the number of hormone-responsive cis-acting elements locating on the promoters of GELP family members in G. raimondii, G. arboreum, and G. hirsutum. Supplementary Table 24. The RT-qPCR primers used to detect the relative expression of GhGELPs during cotton fiber development and in response to plant hormone treatment.

附加文件1：补充表1。本研究中使用的棉属基因组来源。
补充表2。雷蒙德氏棉（G. raimondii）、亚洲棉（G. arboreum）与陆地棉（G. hirsutum）中GELP基因的直系同源/旁系同源基因对。
补充表3。陆地棉GELP基因的核酸编码区与氨基酸序列。
补充表4。雷蒙德氏棉、亚洲棉与陆地棉中GELP基因序列的理化性质分析。
补充表5。雷蒙德氏棉、亚洲棉与陆地棉中GELP基因家族成员的蛋白质保守结构预测分析及潜在功能分类。
补充表6。雷蒙德氏棉、亚洲棉与陆地棉各亚基因组上共线性的GELP同源基因对。
补充表7。雷蒙德氏棉、亚洲棉与陆地棉中GELP直系或旁系同源基因对的序列碱基替换率。
补充表8。雷蒙德氏棉、亚洲棉与陆地棉中不同组织特异性表达的GELP基因的表达水平FPKM值。
补充表9。陆地棉纤维起始与纤维生长阶段GELP基因的表达FPKM值。
补充表10。陆地棉种子萌发过程中不同组织内GELP基因的表达FPKM值。
补充表11。陆地棉中响应非生物胁迫的GELP基因的表达FPKM值。
补充表12。陆地棉中响应大丽轮枝菌（Verticillium dahliae）胁迫的GELP基因的表达FPKM值。
补充表13。纤维发育初始阶段上调与下调表达的GELP基因。
补充表14。纤维发育过程中上调与下调表达的GELP基因。
补充表15。种子萌发初始阶段上调与下调表达的GELP基因。
补充表16。种子萌发后根组织中上调与下调表达的GELP基因。
补充表17。种子萌发后子叶组织中上调与下调表达的GELP基因。
补充表18。响应20%聚乙二醇（PEG）胁迫的上调与下调表达GELP基因。
补充表19。响应4℃低温胁迫的上调与下调表达GELP基因。
补充表20。响应38℃高温胁迫的上调与下调表达GELP基因。
补充表21。经400 mM氯化钠（NaCl）胁迫处理后上调与下调表达的GELP基因。
补充表22。响应大丽轮枝菌胁迫的上调与下调表达GELP基因。
补充表23。雷蒙德氏棉、亚洲棉与陆地棉中GELP家族成员启动子区域激素响应顺式作用元件数量统计。
补充表24。用于检测陆地棉GELP基因在棉花纤维发育过程及植物激素处理下相对表达量的实时定量聚合酶链反应（RT-qPCR）引物。