Drosera rotundifolia induces gene expression changes in human bronchial epithelial cells 16HBE

16HBE cells were incubated for 3 or 6h with a homeopathic preparation of Drosera r. or the solvent control and the transcriptome was analyzed by RNA sequencing. The expression changes of the main statistically significant genes were validated through follow-up experiments using RT-qPCR.Compared with the control solution, Drosera r. changed the expression of dozens of genes already after 3h and this effect was amplified after 6 hours of treatment. The main target genes were some ligands of epithelial growth factor receptor (EREG, AREG, EPGN), genes involved in xenobiotic detoxification (CYP1B1, TIPARP) and chemokines. The network of the main biological functions included epithelial cell proliferation, regulation of angiogenesis, cell chemotaxis, and wound healing.Drosera r. acts on a complex and faceted set of genes, potentially involved in different layers of the bronchial mucosa. The global effect of the plant in 16HBE epithelial cells is a mild stress response that primes the epithelial reparative process and recruits the innate cell defense.

将16HBE细胞以圆叶茅膏菜（Drosera r.）的顺势疗法制剂或溶剂对照分别孵育3小时或6小时，随后通过RNA测序开展转录组分析。通过实时定量聚合酶链反应（RT-qPCR）的后续验证实验，验证了一批具有统计学显著性的核心基因的表达变化。与溶剂对照组相比，圆叶茅膏菜（Drosera r.）在孵育3小时后即可改变数十个基因的表达水平，且该调控效应在处理6小时后进一步增强。其主要靶基因包括表皮生长因子受体的部分配体（EREG、AREG、EPGN）、参与外源性物质解毒的基因（CYP1B1、TIPARP）以及趋化因子。核心生物学功能网络涵盖上皮细胞增殖、血管生成调控、细胞趋化性与伤口愈合。圆叶茅膏菜（Drosera r.）可作用于一组复杂且多维度的基因群，这些基因可能参与支气管黏膜的不同功能层面。该植物在16HBE上皮细胞中诱导的整体效应为轻度应激反应，可启动上皮修复过程并募集固有细胞防御机制。