Phenotypic variation within and across transcriptomic cell types in mouse motor cortex

We used Patch-seq to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of over 1300 neurons in adult mouse motor cortex, providing a comprehensive morpho-electric annotation of almost all transcriptomically defined neural cell types. Contained in this dandiset are the intracellular electrophysiological recordings. See Dandiset #35 for an additional dataset, recorded under the physiological temperature.

本研究采用膜片钳单细胞测序（Patch-seq）技术，整合膜片钳记录、生物胞素染色与单细胞RNA测序，对成年小鼠运动皮层内的1300余个神经元开展分析，实现了对几乎所有转录组定义神经细胞类型的全面形态-电生理注释。本dandiset中收录有胞内电生理记录数据。如需获取在生理温度下记录的额外数据集，请查阅Dandiset #35。