HR-MAS NMR Allied to Chemometric on Hancornia speciosa Varieties Differentiation

This work describes the potential of chemometric analyses applied to 1H high-resolution magic angle spinning nuclear magnetic resonance (1H HR-MAS NMR) data for the chemotaxonomic investigation of Hancornia speciosa (Apocynaceae) varieties. This plant, popularly known as mangaba, has a complex morphological differentiation and thus chemical analyses can be used for their taxonomic classification. In comparison to traditional techniques, 1H HR-MAS NMR allied with chemometrics provided a simple and low cost method for chemotaxonomy. Leaves of four varieties of H. speciosa from a common garden experiment was studied and demonstrated that H. speciosa var. speciosa differs from others due to its specific metabolic profile, and var. pubescens was discriminated based on its high phenolic compound content. The distinction between the latter variety and gardineri is important once it allows for the selection of samples with greater commercial value, once they produce the largest and heaviest fruits.

本研究阐述了化学计量学分析应用于1H高分辨率魔角旋转核磁共振（1H HR-MAS NMR）数据，在香籽木（Hancornia speciosa，夹竹桃科）变种化学分类学研究中的应用潜力。该植物俗称曼加巴（mangaba），其形态分化特征复杂，因此可借助化学分析实现分类鉴定。相较于传统技术，结合化学计量学的1H HR-MAS NMR技术为化学分类学研究提供了一种简便且低成本的方法。本研究对来自同质园实验的4个香籽木变种的叶片开展分析并证实：香籽木原变种（var. speciosa）凭借其独特的代谢谱与其他变种形成区分；柔毛变种（var. pubescens）则因其较高的酚类化合物含量得以区分。而柔毛变种与gardneri变种之间的区分具有重要意义，因其可助力筛选商业价值更高的样本——这类样本能够结出最大且最重的果实。