INHIBITING GENE EXPRESSION IN INSECT PESTS

FIELD: biotechnology. SUBSTANCE: described is a group of inventions comprising a double-stranded ribonucleic acid (dsRNA)， which functions upon absorption by the type of pest insect with inhibition of target gene expression in said insect pest， a DNA construct for expression of said dsRNA， a host cell， a composition for preventing or combating insect attack， use of the composition for preventing and/or combating pest attack， use of the composition as a pesticide for plants， method for inhibiting the expression of a target gene in a type of pest insect in order to prevent and/or combat pest attack， use of a dsRNA， a DNA construct or a composition for preventing or combating an attack of an insect pest， and a kit， containing a dsRNA， a DNA construct or a composition for preventing or combating an attack of an insect pest and one suitable diluent or carrier. EFFECT: invention extends the range of agents for inhibiting expression of a target gene in insect pests. 26 cl， 21 tbl， 6 ex， 21 dwg

技术领域：生物技术领域。技术方案：本系列发明包含一种双链核糖核酸（double-stranded ribonucleic acid，dsRNA），该物质被目标害虫摄取后可抑制该害虫体内的靶基因表达；同时包含用于表达该双链核糖核酸的DNA构建体、宿主细胞、用于预防或防治害虫侵害的组合物；还涵盖该组合物在预防和/或防治害虫侵害中的应用、该组合物作为植物用农药的应用；用于预防和/或防治害虫侵害的、抑制目标害虫体内靶基因表达的方法；双链核糖核酸、DNA构建体或组合物在预防或防治害虫侵害中的应用；以及试剂盒，该试剂盒包含用于预防或防治害虫侵害的双链核糖核酸、DNA构建体或组合物，以及一种合适的稀释剂或载体。有益效果：本发明拓展了用于抑制害虫体内靶基因表达的药剂的范围。本发明包含26项权利要求、21张表格、6个实施例及21幅附图。