ExtFig7C_qPCR_STOML2-YKT6-mut-int_R1_LG322.xlsx

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters with 99 bp insertions from the STOML2 and YKT6 genes in the intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA and 18S rRNA levels were quantified by qRT-PCR

HEK293T ishXrn1细胞经多西环素（doxycycline）处理3~4天以诱导Xrn1基因敲低，随后将内含子区插入了STOML2与YKT6基因来源的99 bp片段的荧光素酶报告载体转染至细胞，同时设置是否共转染PR8 PA-X的组别。提取细胞总RNA后，通过实时荧光定量逆转录PCR（qRT-PCR）定量检测荧光素酶mRNA与18S核糖体RNA（18S rRNA）的表达水平。