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Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIP-Seq)

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NIAID Data Ecosystem2026-03-14 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP377978
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The response of endothelial cells to diabetic-simulating stimuli is poorly understood. To evaluate the changes of enhancer activation status in HUVEC, genome-wide enrichment of H3K27ac (marker of enhancer and open chromatin) was examined in HUVECs treated with combined high glucose and TNF-alpha. HUVECs incubated in 25 mM mannitol were used as osmolarity control. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27ac in HUVEC treated by 25 mM glucose and 5 ng/ml TNFa for 3 and 7 days
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2023-02-09
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